Imaging of SPIO Labelled Endothelial Networks at 3, 7 and 11.7 Tesla
نویسندگان
چکیده
Introduction Endothelial progenitor cells (EPC) are promising candidates for novel stem cell transplantation strategies to treat cardiovascular diseases. Within this study formations of vascular network structures with super paramagnetic iron oxide (SPIO) loaded EPCs were induced, which is an in vitro correlate of the vasculogenesis process [1]. The resulting endothelial network showed to be an optimal candidate to optimize labelling strategies as well as protocols for single cell detection. High resolution (55μm) images at 3, 7 and 11.7 Tesla systems were investigated and the impact of different field strength and gradient performance on the image quality was examined.
منابع مشابه
Behaviour of adipose-derived canine mesenchymal stem cells after superparamagnetic iron oxide nanoparticles labelling for magnetic resonance imaging
BACKGROUND Therapy with mesenchymal stem cells (MSCs) has been reported to provide beneficial effects in the treatment of neurological and orthopaedic disorders in dogs. The exact mechanism of action is poorly understood. Magnetic resonance imaging (MRI) gives the opportunity to observe MSCs after clinical administration. To visualise MSCs with the help of MRI, labelling with an MRI contrast ag...
متن کاملEfficient nano iron particle-labeling and noninvasive MR imaging of mouse bone marrow-derived endothelial progenitor cells
In this study, we sought to label mouse bone marrow-derived endothelial progenitor cells (EPCs) with Resovist(®) in vitro and to image them using 7.0 Tesla (T) magnetic resonance imaging (MRI). Mouse bone marrow-derived EPCs were cultured in endothelial basal medium with endothelial growth supplement. They were then characterized by immunocytochemistry, flow cytometry, and fluorescence quantita...
متن کاملThe effects of iron oxide incorporation on the chondrogenic potential of three human cell types
Non-invasive monitoring of living cells in vivo provides an important tool in the development of cell-based therapies in cartilage tissue engineering. High-resolution magnetic resonance imaging (MRI) has been used to monitor target cell populations in vivo. However, the side-effects on cell function of the labelling reagents, such as superparamagnetic iron oxide (SPIO), are still unclear. This ...
متن کاملUTE Imaging for Single Cell Detection with Positive Contrast
Introduction Employing gradient recalled echo (GRE) techniques to image cells labelled with super paramagnetic iron oxides (SPIO) is known to be an efficient method for the detection of small cell quantities in-vivo [1]. Dephasing within a voxel, and therefore negative contrast of the pixel, depends on the local magnetic dose within this voxel [2] and the global field-distortions caused by SPIO...
متن کاملImaging islets labeled with magnetic nanoparticles at 1.5 Tesla.
We have developed a magnetic resonance imaging (MRI) technique for imaging Feridex (superparamagnetic iron oxide [SPIO])-labeled islets of Langerhans using a standard clinical 1.5-Tesla (T) scanner and employing steady-state acquisition imaging sequence (3DFIESTA). Both porcine and rat islets were labeled with SPIO by a transfection technique using a combination of poly-l-lysine and electropora...
متن کامل